Packet 5: Tossup 8

Rotation is induced by this technique’s “pulsed-field” form. (15[2]-5[1])The products of this technique are placed onto a nitrocellulose membrane during (15[1])Southern (15[1])blotting. (15[5]-5[1])Uneven (15[1])heating (15[2]-5[1])in this technique results in the appearance (15[2])of a “smiling” (-5[1])effect. (15[1])Isoelectric (15[2])focusing precedes a form of this technique (15[1])in its (15[1])“two-dimensional” (15[1])variant. (15[1])In order (-5[1])to (-5[1])visualize this technique’s results under (*) UV light, (-5[1])a standard (10[1])reference called a “ladder” (10[2])may be placed in (10[1])the first (10[2])well. (10[2]-5[1])This technique (10[1])is often (10[1])run inside polyacrylamide (10[1]-5[1])(“poly-uh-KRILL-uh-mide”) or agarose (10[1])(“AH-guh-rohss”). For 10 points, name this technique (10[1])that uses an electric field (10[2])to separate DNA (10[1])fragments by their size inside of a gel. (10[1])■END■ (10[3]0[3])

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