Round 5: Tossup 8

Rotation is induced by this technique’s “pulsed-field” form. (15[1])The products of this technique are placed onto a nitrocellulose membrane during Southern blotting. (15[2]-5[1])Uneven heating (15[1])in this technique results in the appearance of a “smiling” (-5[1])effect. Isoelectric focusing precedes a form of this technique in its “two-dimensional” (15[1])variant. In order to visualize this technique’s results under (*) UV light, a standard reference called a “ladder” may be placed in the first well. This technique is often run inside polyacrylamide (“poly-uh-KRILL-uh-mide”) or agarose (“AH-guh-rohss”). For 10 points, name this technique (10[1])that uses an electric field to separate DNA fragments by their size inside of a gel. ■END■

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