Round 5: Tossup 8

Rotation is induced by this technique’s “pulsed-field” form. The products of this technique are placed onto a nitrocellulose membrane during Southern blotting. Uneven heating in this technique results in the appearance (15[1])of a “smiling” effect. Isoelectric focusing precedes a form of this technique in its “two-dimensional” variant. In order to visualize this technique’s results under (*) UV light, (-5[1])a standard reference called a “ladder” may be placed in the first well. This technique is often run inside polyacrylamide (“poly-uh-KRILL-uh-mide”) or agarose (“AH-guh-rohss”). For 10 points, name this technique that uses an electric field to separate DNA fragments by their size inside of a gel. ■END■ (0[2])

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