Round 5: Tossup 8

Rotation is induced by this technique’s “pulsed-field” form. (15[1])The products of this technique are placed onto a nitrocellulose membrane during (15[1])Southern blotting. Uneven heating in this technique results in the appearance of a “smiling” effect. Isoelectric focusing precedes a form of this technique in its “two-dimensional” variant. In order to visualize this technique’s results under (*) UV light, a standard (10[1])reference called a “ladder” (10[1])may be placed in (10[1])the first well. This technique is often run inside polyacrylamide (“poly-uh-KRILL-uh-mide”) or agarose (“AH-guh-rohss”). For 10 points, name this technique that uses an electric field to separate DNA fragments by their size inside of a gel. ■END■

ANSWER: gel electrophoresis [accept SDS-PAGE; accept subtypes like agarose gel electrophoresis; accept two-dimensional gel electrophoresis or pulsed-field gel electrophoresis]
<Science - Biology, Spencer Manning> | VAULT II Packet 05
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