Round 5: Tossup 8

Rotation is induced by this technique’s “pulsed-field” form. The products of this technique are placed onto a nitrocellulose membrane during Southern blotting. Uneven heating in this technique results in the appearance of a “smiling” effect. Isoelectric focusing precedes a form of this technique in its “two-dimensional” variant. In order (-5[1])to visualize this technique’s results under (*) UV light, a standard reference called a “ladder” (10[1])may be placed in the first (10[1])well. This technique is often (10[1])run inside polyacrylamide (“poly-uh-KRILL-uh-mide”) or agarose (“AH-guh-rohss”). For 10 points, name this technique that uses an electric field to separate DNA fragments by their size inside of a gel. ■END■ (0[1])

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